Subjects: Medicine, Pharmacy >> Traditional Chinese Medicine and Chinese Materia Medica submitted time 2020-05-05
Gao, Yuan
Yu, Wanxin
Duan, Xiaomei
Ni, Lianli
Liu, Heng
Zhao, Hairong
Xiao, huai
Zhang, Chenggui
Yang, Zhibin
Abstract： Rheumatoid arthritis (RA) is an autoimmune disease. Wasp venom (WV) relieves rheumatoid arthritis considered as a traditional folk medicine in Jingpo nationality in Yunnan, China. The current study aimed to investigate the effect of wasp venom ameliorating rheumatoid arthritis symptoms in experimental rats. We established a model of type II collagen (CII)-induced arthritis (CIA) in SD rats and examined the inhibition of inflammation and autoimmune response. The anti-arthritic effects of WV were evaluated through the paw swelling, histopathological score and histopathology changes of the affected paw were assessed. The anti-inflammation effects were assayed by the level of IL-6, TNF-α, IL-1β and the number of inflammatory cells in peripheral blood. The alteration of the T cell subsets ratio in the spleen of rats was detected by ?ow cytometry, at the same time the viscera index and immune serum globulin levels were evaluated. The results suggested that various doses of WV (0.125, 0.25 and 0.5 mg/kg) significantly alleviated paw swelling and arthritis score in CIA rats with the untreated control (P < 0.05). WV (0.25 and 0.5 mg/kg) relieved synovial tissue lesions of ankle joints and histopathology scores of synoviocyte hyperplasia and inflammatory cell infiltration with vehicle group (P < 0.05). Regarding immunological regulation, 0.5 mg/kg WV lowered the immune serum globulin levels (P < 0.05), and we further found that WV (0.5 mg/kg) suppressed the immune response of Th cells, while enhanced the functions of Tc cells and Treg cells in spleen cells markedly (P < 0.05). The immunosuppressive action of WV displayed was analogous to its inhibitory effect on IL-1β, TNF-α, IL-8, IL-6, COX-2 and PGE2 levels in rat serum. In conclusion, these findings demonstrated that WV exhibited anti-arthritic activity, which might be associated with their inhibitory effects on immunoregulation and anti-inflammatory action."
Peer Review Status:
Awaiting Review
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